UW Cytogenetic Services: General

C-Banding (constitutive heterochromatin banding)

Constitutive heterochromatin includes repetative DNA, satellite DNA, and some non-repetative DNA and is found near the centromemeres and at the end of the long arm of the Y in human chromosomes. These regions stain darkly after treatment of the slide with acid, heat and alkyline solutions followed by staining with Leishman stain.

Notice the large heterochromatic regions on chromosomes 1 and 9.

Procedure

Wisconsin State Laboratory of Hygiene

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