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C-Banding (constitutive heterochromatin banding)


Constitutive heterochromatin includes repetative DNA, satellite DNA, and some non-repetative DNA and is found near the centromemeres and at the end of the long arm of the Y in human chromosomes. These regions stain darkly after treatment of the slide with acid, heat and alkyline solutions followed by staining with Leishman stain.


Notice the large heterochromatic regions on chromosomes 1 and 9.

Procedure

 
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The University of Wisconsin Cytogenetic Services Laboratory is affiliated with the Waisman Center, the University of Wisconsin Comprehensive Cancer Center, and the Wisconsin State Laboratory of Hygiene
 


UW Cytogenetic Services

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Madison WI 53706
Phone: 1-608-262-0402
Fax: 1-608-265-7818

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