FISH: (Fluorescent In Situ Hybridization).
FISH is the detection of highly specific DNA probes which have been hybridized to either interphase or metaphase chromosomes using fluorescence microscopy.
DNA for probe use is labeled with fluorescent (direct method) or non fluorescent molecules which are then detected by fluorescent antibodies (indirect method). The probes bind to a specific region or regions on the target chromosome. The chromosomes are then stained using a contrasting color, and the cells are viewed using a fluorescence microscope.
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