Silver (NOR) staining

SILVER STAINING OF
NUCLEOLAR ORGANIZING REGIONS (NOR)

Regions within the "stalks" of chromosomes 13,14,15,21, and 22 (the acrocentric chromosomes) contain genes for 18S and 28S rRNA. The silver nitrate stain used in this method binds to proteins near these regions during active transcription. The staining intensity varies from person to person and chromosome to chromosome.

Visible are eight of the ten acrocentric chromosomes with their stalk regions staining darkly.
Procedure

Wisconsin State Laboratory of Hygiene

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