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Flow Cytometry E. coli O157:H7
E. coli O157:H7 is a highly virulent pathogen implicated in food and waterborne outbreaks. Documented outbreaks include over 10,000 cases with several hundred deaths. Existing methods for detection of E. coli O157:H7 in water are non-standardized, have poor sensitivity, or require enrichment steps resulting in assay times exceeding 72 hours. The WSLH has developed a rapid flow cytometric assay capable of attaining presumptive positive results in less than 8 hours. Presumptive positives are confirmed using culture and PCR. The assay also improves sensitivity down to 10 organisms/500 mL, an acceptable level for public health investigations. Please note that this test is offered as a research tool only. 2003 Poster by Marty Collins (WSLH) for ASM on E. Coli 0157 Work (660 KB, Powerpoint File) |
