Flow Cytometry

Research and Publications

Research Activities

The Laboratory is involved in a variety of internally and externally funded projects including development of monoclonal antibodies to anatoxin A, improving methods to detect Mycobacterium avium complex organisms in finished water and biofilms, pathogen removal and inactivation studies, evaluation of products and reagents to detect environmental pathogens, pathogen surveillance studies, and improvement of methods to detect waterborne pathogens. A list of the more recent publications generated by the Laboratory is listed below:

Publications and Presentations

Hoffman, R.M. Wolk, D. M., Spencer, S.K., and M. Borchardt. Development of methods for detection of waterborne microsporidia. (in preparation).

Lau, B., Harrington, G.W., Hoffman, R.M., and M.A. Borchardt. 2005. L T2 Crypto Removal Credits: Are They Good Enough? Proceedings of the 2005 Water Quality Technology Conference, Quebec City, Canada.

Wareing D.R.A., Sarian T., Duncanson P., Pearson J., Hoffman, R., Collins, M., and C. Powell. 2005. Evaluation of Dynabeads Anti-Legionella® for the isolation and quantification of Legionella from water samples. Proceedings of the 2005 Water Quality Technology Conference, Quebec City, Canada.

Hoffman, R.M. and M.M. Marshall. 2004. Microbes Continue to Pose a Detection Challenge. Journ. AWWA 96(8):66-68.

Plummer, C., Hoffman, R., Sturbaum, G., Marshall, M., Sterling , C., and D. M. Wolk. 2004. Multiplex Real-time PCR for Encephalitozoon Spores Includes Monitor for PCR Inhibitors in Water Samples. 104th Meeting of the American Society for Microbiology. Q-101: New Orleans, LA.

Hoffman R.M., Marshall M.M., Polchert D.M. and B.H. Jost. 2003. Identification and Characterization of Multiple Subpopulations Within Cell Culture-Propagated, Percoll Purified Encephalitozoon intestinalis Spore Suspensions. Appl. Environ. Microbiol. 69(8):4966-70.

Collins, M.J., Hoffman, R.M., Argue, B.T., and J.H. Standridge. 2003. Development of a Rapid Detection Method for Waterborne E. coli O157:H7. 103 rd Meeting of the American Society for Microbiology. Poster Q496. Washington, DC.

Hoffman R., Marshall M., Polchert D., Spencer S., Moffet J., and M. Borchardt. 2002. Concentration and Detection of Microsporidia Using a Newly-Modified Continuous Flow Centrifuge and Flow Cytometry. Proceedings of the 2002 Water Quality Technology Conference, Seattle WA.

Marshall M., Moffett J., Hoffman R., Sturbaum G., Sterling C., and B.H. Jost. 2002. Encephalitozoon spp. Detection and Viability Determined by RT-PCR Cell Culture Assays Using Primer Pairs from 3 Distinct Genes. Proceedings of the 2002 Water Quality Technology Conference, Seattle WA .

Marshall M., Hoffman R., Moffett J., Jost B.H., Ware M., Sturbaum G., and D. Battigelli. 2002. Identification and Characterization of Infectious and Non-Infectious Sub-Populations of Encephalitozoon intestinalis Spores Purified from in vitro Cell Culture. Proceedings of the International Symposium on Waterborne Pathogens. Cascais/Lisbon, Portugal 2002.

Hoffman R.M., Chauret C., Standridge J.H., and L. Peterson. 1999. Evaluation of four commercial antibodies. Jour. Amer. Water Works Assoc. 91(9):69-78.

Hoffman R.M., Chauret C.C., Standridge J.H., and L. Peterson. 1998. Evaluation of antibodies to Cryptosporidium and Giardia using flow cytometry. In Proc. Water Quality Technology Conference. San Diego, CA.

Hoffman R.M., Standridge J.H., Prieve A.F., Cucunato J.C., and M. Bernhardt. 1997. Using flow cytometry to detect protozoa. Jour. Amer. Water Works Assoc. 89(9):104-111.

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