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WSLH Toxicology: Arsenic Testing

Arsenic Testing is performed on urine and hair samples. Urine is the preferred sample for measuring recent exposures, while hair analysis is primarily used for confirming chronic toxicity. Some common environmental sources of arsenic exposure include seafood, well water, arsenic treated wood, and arsenic containing pesticides.

Specimen collection:

Urine: 10 mL aliquot of a 24-hour collection is the preferred specimen; a random specimen is acceptable. A Urine Toxicology Kit (Kit #47) can be used for sending the specimen to us.
Hair: 30 mg specimen required for single analysis. A lock about the size of a cocktail straw should be cut at the scalp, and be a minimum of 1 inch in length. Shorter hair should come from a proportionately larger size lock. Mark the proximal end using a string or similar tie. Place in a vial or ziplock bag labeled with the patient's name for shipment.

Testing Procedure:

The biological samples are digested and the arsenic is oxidized to the pentavalent form. The arsenic is converted to the iodide and extracted into toluene. The arsenic complex is then back-extracted into an acidic nickel nitrate solution and the arsenic measured by graphite furnace atomic absorption spectroscopy.

Links:

Wisconsin Department of Natural Resources Arsenic in Drinking Water and Groundwater Information Page
Link to EHD water testing
The Agency for Toxic Substances & Disease Registry (ATSDR)

 
Wisconsin State Laboratory of Hygiene
 
Clinical Laboratories
465 Henry Mall
Madison WI 53706
Ph: 608-262-1293
1-888-494-4324
Fax: 1-608-262-3257
Environmental Laboratories
2601 Agriculture Drive
Madison WI 53718
Ph: 608-224-6202
1-800-442-4618
Fax: 1-608-224-6213
Walton Commons
2810 Walton Commons West
Madison, WI 53718
Help Desk: 608-221-6262
Fax: 1-608-221-6297

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