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WSLH Toxicology: Arsenic Testing
Arsenic Testing is performed on urine and hair samples. Urine is the preferred sample for measuring recent exposures, while hair analysis is primarily used for confirming chronic toxicity. Some common environmental sources of arsenic exposure include seafood, well water, arsenic treated wood, and arsenic containing pesticides.
Specimen collection:
| Urine: |
10 mL aliquot of a 24-hour collection is the preferred specimen; a random specimen is acceptable. A Urine Toxicology Kit (Kit #47) can be used for sending the specimen to us. |
| Hair: |
30 mg specimen required for single analysis. A lock about the size of a cocktail straw should be cut at the scalp, and be a minimum of 1 inch in length. Shorter hair should come from a proportionately larger size lock. Mark the proximal end using a string or similar tie. Place in a vial or ziplock bag labeled with the patient's name for shipment. |
Testing Procedure:
The biological samples are digested and the arsenic is oxidized to the pentavalent form. The arsenic is converted to the iodide and extracted into toluene. The arsenic complex is then back-extracted into an acidic nickel nitrate solution and the arsenic measured by graphite furnace atomic absorption spectroscopy.

Links:
Wisconsin Department of Natural Resources Arsenic in Drinking Water and Groundwater Information Page Link to EHD water testing The Agency for Toxic Substances & Disease Registry (ATSDR)
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