NPM1 Mutation Analysis

NPM1 Mutation Analysis
WSLH Department: Cytogenetics
WSLH Test Code:895M61
CPT Code: 
Price: 
Includes:NPM1 exon 12 Mutation Analysis. Testing is performed by Blood Center of Wisconsin. Interpretive comments provided by UW Cytogenetic Services and Molecular Diagnostics.
Methodology:**(CPT Code: 81310)** NPM1 exon 12 mutations are detected by PCR amplification with fluorescently labeled primers and capillary electrophoresis-based fragment analysis.
Availability:Monday-Friday 7:45 AM - 4:30 PM, Saturday 7:45 AM - 12:00 PM
Turn-around Time:Approximately 10-14 days. (Reports are issued Monday-Friday 7:45 AM - 4:30 PM)
Recommended Uses:Risk stratification in patients with cytogenetically normal acute myeloid leukemia (CN-AML).
Contraindications: 
Specimen Requirements:**10 mL whole blood collected in EDTA (purple top) tube
**2-5 mL bone marrow collected in EDTA tube
**High Quality DNA (60ul) >= 1.5 ug at 25 ng/ul
Collection Kit/Container: 
Patient Preparation: 
Collection Instructions:Draw blood using aseptic techniques into a sterile EDTA vacuum type tube(s). Invert tube(s) to mix. If using larger tubes, draw to full volume to avoid over-treatment with anticoagulant.
Specimen Handling and Transport:Store and transport specimens at room temperature (may transport with coolant during hot, >85 degrees, weather). DO NOT FREEZE. Specimens must be received by the laboratory within 24-48 hours of collection.
Unacceptable Conditions:Blood or bone marrow that is clotted or hemolyzed is not acceptable. Blood or bone marrow must not be frozen. Plasma and serum are not acceptable.
Requisition Form:Cytogenetics Lab Neoplasia Diagnosis Form #132
Required Information:Laboratory regulations require the following minimum information to be provided on the requisition form for a specimen to be accepted for testing: Patient name or unique identifier; date and time of collection, patient date of birth and sex, specimen type/site of collection, test request(s), reason for referral, clinician name and UPIN/NPI, and address for reporting results. Please be certain that name/identifier on the form matches that on the specimen label.
Results include:NPM1 exon 12 length variant detected/not detected
Limitations:This assay will only detect known length mutations in NPM1 exon 12. Rare mutations in other exons will not be detected. The lower limit of detection of the NPM1 assay is approximately 5%.
Additional Tests Recommended:*AML FISH panel, Chromosome analysis- Bone Marrow for Hematologic Disorders,
Chromosome analysis- Unstimulated Blood for Hematologic Disorders
Additional Comments: 
Additional Tests Performed: 

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