Chromosome Analysis, Bone Marrow, for Hematologic Disorders

Chromosome Analysis, Bone Marrow, for Hematologic Disorders
WSLH Department: Cytogenetics
WSLH Test Code:812
Includes:G-banded chromosome analysis of unstimulated, spontaneously dividing, bone marrow cells. Includes short term culture of bone marrow, examination of at least 20 metaphase cells (when possible), and preparation of two karyograms. Additional cells may be examined if indicated by initial results. Additional karyograms may be prepared if multiple cell lines are observed. Quality and number of cells analyzed may be limited if specimen is not adequate.
Methodology:**(CPT Codes: 88237, 88264)** Microscopic analysis of G-banded chromosomes.
Availability:Monday-Friday 7:45 AM - 4:30 PM, Saturday 7:45 AM - 12:00 PM
Turn-around Time:Approximately 6-7 days, RUSH specimens reported by phone or fax in 3-4 days. (Reports are issued Monday-Friday, 7:45 AM - 4:30 PM)
Recommended Uses:Identification of chromosome abnormalities associated with hematologic disorders such as Acute Myeloid Leukemia (AML), Acute Lymphoid Leukemia (ALL), lymphoma, myeloma. Aid in diagnosis and follow-up after treatment or bone marrow transplant.
Specimen Requirements:1-2 ml early aspirate of bone marrow collected in sodium heparin.
Collection Kit/Container: 
Patient Preparation: 
Collection Instructions:1.0-2.0ml bone marrow from 1st or 2nd aspirate. Material submitted for cytogenetic analysis must be from an early aspirate to avoid dilution with blood. Draw sodium heparin solution (1000 USP units/ml) into the syringe to be used for aspiration and then expel (over-heparinization is toxic to the cells). Transfer the marrow to a sterile, Na-Heparin vacuum type tube for delivery to State Lab. If an aspirate is unsuccessful, a 10mm bone core biopsy may be submitted in a sterile transport medium with Na-Heparin.
Specimen Handling and Transport:Store and transport specimens at room temperature (may transport with coolant during hot, >85 degrees F weather). DO NOT FREEZE. The laboratory must receive specimens within 24 hours of collection.
Unacceptable Conditions:Bone marrow that is collected in EDTA, sodium citrate, or other anti-coagulants is not acceptable. Bone marrow that is clotted or hemolyzed is not acceptable. Bone marrow must not be frozen.
Requisition Form:Cytogenetics Lab Neoplasia Diagnosis Form #132
Required Information:Laboratory regulations require the following minimum information to be provided on the requisition form for a specimen to be accepted for testing: Patient name or unique identifier; date and time of collection, patient date of birth and sex, specimen type/site of collection, test request(s), reason for referral, clinician name and UPIN/NPI, and address for reporting results. Please be certain that name/identifier on the form matches that on the specimen label.
Results include:Karyotype written using International System for Human Cytogenetic Nomenclature (ISCN) 2013 and interpretation of results.
Limitations:Analysis is dependent on the presence of actively dividing cells in the bone marrow specimen.
Additional Tests Recommended:Fluorescence in situ hybridization analysis may be recommended to characterize chromosome abnormalities; if performed there will be an additional charge.
Additional Comments: 
Additional Tests Performed: 

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