Prothrombin Gene Mutation by PCR [PTPCR]

Prothrombin Gene Mutation by PCR [PTPCR]
WSLH Department: Cytogenetics
WSLH Test Code:842C92
CPT Code: 
Price: 
Includes:est Component:
Prothrombin (G20210A) gene mutation analysis by real-time PCR amplification and melting point analysis.
Detect presence of Prothrombin G20210A allele, which has been associated with elevated plasma prothrombin levels and moderately increased risk of first venous thrombotic events. Usually ordered in conjunction with Factor V Gene Mutation Analysis. The mutation of the prothrombin G20210A gene in the heterozygous state can confer an approximately 3 fold increased life-long risk for venous thrombosis. Due to low frequency, the G20210A mutation in the homozygous state and the additive effects of the G20210A mutation in combination with the Factor V mutation remains undetermined. Usually ordered with Prothrombin mutation is a test for Factor V Leiden mutation, which has been used to confirm the diagnosis of resistance to APC.
Methodology:**(CPT Code: 81240)** Real-Time PCR followed by Melting Point Analysis
Availability:Once a week.
Turn-around Time:10 Days
Recommended Uses:Blood
Contraindications: 
Specimen Requirements:4 mL, (2 mL pediatric)
Lavender top EDTA,
Also Acceptable: Light blue top (3.2% NaCitrate)
Collection Kit/Container: 
Patient Preparation: 
Collection Instructions:Stability Ambient:
7 days,
Stability Refrigerated:
3 weeks
Specimen Handling and Transport:Transport specimen to Laboratory immediately. Refrigerate specimen if not delivered immediately. Transport on coolant pack if coming from clinic location; avoid freezing. Specimen must be received within 7 days of collection date.
Unacceptable Conditions:Frozen:
Not acceptable
Requisition Form:Cytogenetics Lab Genetic Diagnosis Form #131
Required Information: 
Results include:A written interpretive report is provided by the laboratory.
Limitations: 
Additional Tests Recommended: 
Additional Comments:This test was performed using a modification of an FDA approved method. The performance characteristics of this test were determined by the UWHC Clinical Laboratories and meet the standards for clinical testing.
Additional Tests Performed: 

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