Chromosome Analysis, Amniotic Fluid-Abridged, for Pre-natal Genetic Diagnosis

Chromosome Analysis, Amniotic Fluid-Abridged, for Pre-natal Genetic Diagnosis
WSLH Department: Cytogenetics
WSLH Test Code:852
CPT Code: 
Price: 
Includes:G-banded chromosome analysis of cultured amniocytes. Includes in situ culture of cells from amniotic fluid, examination of 5 metaphase cells from 5 independent colonies, and preparation of 1 karyogram.
Methodology:**(CPT Codes: 88235, 88261)** Microscopic analysis of G-banded chromosomes.
Availability:Monday-Friday 7:45 AM - 4:30 PM, Saturday 7:45 AM - 12:00 PM
Turn-around Time:Approximately 7-10 days (longer for specimens with small volume, containing blood or slow growth). (Reports are issued Monday-Friday 7:45 AM - 4:30 PM)
Recommended Uses:Abridged chromosome analysis to be ordered in conjunction with Prenatal Microarray (Comprehensive or Targeted) for a fetus with one or more major structural abnormalities identified on ultrasonographic examination.
Contraindications: 
Specimen Requirements:15-30 ml amniotic fluid (will be used for both the Abridged chromosome analysis and the Prenatal Microarray)
Collection Kit/Container: 
Patient Preparation: 
Collection Instructions:Collect the fluid under sterile, ultrasound guided conditions using a 22-gauge needle inserted through the uterine wall and into the amniotic cavity. Discard the first 1-2ml of amniotic fluid to minimize the possibility of maternal cell contamination, and dispense 15-30 ml of the remaining fluid into two or three sterile 15 ml centrifuge tubes.
Specimen Handling and Transport:Store and transport specimens at room temperature (may transport with coolant during hot, >85 degrees F weather). DO NOT FREEZE. The laboratory must receive specimens within 24 hours of collection.
Unacceptable Conditions:Sample must not be frozen.
Requisition Form:Cytogenetics Lab Genetic Diagnosis Form #131
Required Information:Laboratory regulations require the following minimum information to be provided on the requisition form for a specimen to be accepted for testing: Patient name or unique identifier; date and time of collection, patient date of birth and sex, specimen type/site of collection, gestational age, test request(s), reason for referral, clinician name and UPIN/NPI, and address for reporting results. Please be certain that name/identifier on the form matches that on the specimen label.
Results include:Karyotype written using International System for Human Cytogenetic Nomenclature (ISCN) 2013 and interpretation of results.
Limitations:The cytogenetic methods used in this analysis do not routinely detect small structural rearrangements, microdeletions, or low level (<38%) mosaicism. Viable cells may be maternally derived.
Additional Tests Recommended:Alpha Fetoprotein quantification (Test 851) in amniotic fluid will be performed for all specimens with a gestational age between 12 and 24 weeks. Includes Acetylcholinesterase (ACHe) quantification when appropriate.
Additional Comments:Prenatal Microarray. Submission of parental blood samples (4ml Sodium Heparin) is recommended to expedite follow-up testing if necessary to characterize abnormal findings in the Prenatal Microarray.
Additional Tests Performed: 

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