Chromosome Analysis, Chorionic Villus Sample-Abridged, for Pre-natal Genetic Diagnosis

Chromosome Analysis, Chorionic Villus Sample-Abridged, for Pre-natal Genetic Diagnosis
WSLH Department: Cytogenetics
WSLH Test Code:857
Includes:G-banded chromosome analysis of cultured cells from chorionic villus sample (CVS). Includes in situ culture of cells from chorionic villus, examination of 5 metaphase cells from 5 independent colonies, and preparation of 1 karyogram.
Methodology:**(CPT Codes: 88235, 88261)** Microscopic analysis of G-banded chromosomes.
Availability:Monday-Friday 7:45 AM - 4:30 PM, Saturday 7:45 AM - 12:00 PM
Turn-around Time:Approximately 8-10 days (longer for specimens with small volume). (Reports are issued Monday-Friday 7:45 AM - 4:30 PM)
Recommended Uses:Abridged chromosome analysis to be ordered in conjunction with Prenatal Microarray (Comprehensive or Targeted) for a fetus with one or more major structural abnormalities identified on ultrasonographic examination.
Contraindications: 
Specimen Requirements:10-30mg chorionic villus sample (will be used for both the Abridged chromosome analysis and the Prenatal Microarray)
Collection Kit/Container:Cytogenetics and Molecular Genetics Collection Kit
Patient Preparation: 
Collection Instructions:Using aseptic technique, obtain at least 10mg of chorionic villi, taken between 11-38 weeks of gestation. Place villi into a flask with transport media provided by the department.
Specimen Handling and Transport:Store and transport specimens at room temperature (may transport with coolant during hot, >85 degrees F, weather). DO NOT FREEZE. Specimens must be received by the laboratory within 24 hours of collection.
Unacceptable Conditions:A specimen with no fetal material identified and only maternal decidua present will be rejected.
Requisition Form:Cytogenetics Lab Genetic Diagnosis Form #131
Required Information:Laboratory regulations require the following minimum information to be provided on the requisition form for a specimen to be accepted for testing: Patient name or unique identifier; date and time of collection, patient date of birth and sex, specimen type/site of collection, gestational age, test request(s), reason for referral, clinician name and UPIN/NPI, and address for reporting results. Please be certain that name/identifier on the form matches that on the specimen label. Karyotype written using International System for Human Cytogenetic Nomenclature (ISCN) 2013 and interpretation of results.
Results include:Karyotype written using International System for Human Cytogenetic Nomenclature (ISCN) 2013 and interpretation of results.
Limitations:The cytogenetic methods used in this analysis do not routinely detect small structural rearrangements, microdeletions, or low level (<38%) mosaicism.
Additional Tests Recommended:Prenatal Microarray. Submission of parental blood samples (4ml Sodium Heparin) is recommended to expedite follow-up testing if necessary to characterize abnormal findings in the Prenatal Microarray.
Additional Comments: 
Additional Tests Performed: 

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