DNA Isolation and Banking

DNA Isolation and Banking
WSLH Department: Cytogenetics
WSLH Test Code:862
Includes:Isolation of genomic DNA from specimen and long-term storage of DNA.
Methodology:**(CPT Code: 81479)** Extraction of whole genomic DNA and long term storage at -80 degrees. Red blood cells are first lysed to separate them from WBCs. DNA is isolated from WBCs by lysing the cells with an anionic detergent in the presence of a DNA stabilizer which limits the activity of DNases. Contaminating RNA is then removed by treatment with RNase I. Other contaminants, such as proteins are removed by salt precipitation. Finally, high molecular weight genomic DNA is recovered by precipitation with isopropanol and resolubilized in a buffered solution containing a DNA stabilizer.
Availability:Monday-Friday 7:45 AM - 4:30 PM, Saturday 7:45 AM - 12:00 PM
Turn-around Time:Approximately 2-4 days. (Reports are issued Monday-Friday 7:45 AM - 4:30 PM)
Recommended Uses:Specimens can be used for analysis of genomic DNA sequence by several different methods including but not limited to: PCR based methods, Southern Blotting, restriction digest, and DNA sequencing.
Contraindications: 
Specimen Requirements:6 ml (preferred) - 2 ml (minimum) of peripheral blood in sterile EDTA (purple top) vacuum type tubes. Extractions will be attempted on all submitted specimens.
Collection Kit/Container: 
Patient Preparation: 
Collection Instructions:Draw blood using aseptic techniques into a sterile EDTA vacuum type tube(s). Invert tube(s) to mix. If using larger tubes, draw to full volume to avoid over-treatment with anticoagulant.
Specimen Handling and Transport:Store and transport specimens at room temperature (may transport with coolant during hot, >85 degrees F weather). DO NOT FREEZE. The laboratory must receive specimens within 24-48 hours of collection.
Unacceptable Conditions:Blood must not be frozen. Plasma and serum are not acceptable. Sample must not be processed with formalin.
Requisition Form:DNA Banking
Required Information:DNA Banking Request Form. Payment of $90 fee. Laboratory regulations require the following minimum information to be provided on the requisition form for a specimen to be accepted for testing: Patient name or unique identifier; date of collection, patient date of birth and sex, specimen type/site of collection, test request(s). Please be certain that name/identifier on the form matches that on the specimen label.
Results include:Amount of DNA extracted from specimen available for future testing.
Limitations: 
Additional Tests Recommended: 
Additional Comments:Forms that can be downloaded from http://www.slh.wisc.edu/clinical/cytogenetics/ include DNA Banking Request Form, DNA Banking Policy Statement, Request for Release of Specimen, and Transfer of Ownership
Additional Tests Performed: 

Back to test list

 

Search Again