Stillbirth Aneuploidy Panel, FISH, Paraffin embedded fetal tissue

Stillbirth Aneuploidy Panel, FISH, Paraffin embedded fetal tissue
WSLH Department: Cytogenetics
WSLH Test Code:875
Includes:FISH analysis of paraffin embedded placenta or fetal tissue to determine copy number of chromosomes 13, 18, 21, X, and Y.
Methodology:**(CPT Codes: 88271x5, 88275)** Fluorescence in situ hybridization using DNA probes for chromosomes 13, 18, 21, X, and Y (AneuVysion Prenatal Test, Abbott Molecular)
Availability:Monday-Friday 7:45 AM - 4:30 PM, Saturday 7:45 AM - 12:00 PM
Turn-around Time:Approximately 10-14 days. (Reports are issued Monday-Friday 7:45 AM - 4:30 PM)
Recommended Uses:Stillbirth or spontaneous miscarriages where chromosome analysis is unsuccessful.
Contraindications: 
Specimen Requirements:Paraffin embedded tissue (FFPE): Five 4-micron sections on immunohistochemistry slides, the middle section stained with H&E
Collection Kit/Container: 
Patient Preparation: 
Collection Instructions:FFPE tissue: Tissue specimen should be fixed in 10% neutral buffered formalin for 6-48 hours.
Specimen Handling and Transport:Store and transport specimens at room temperature (may transport with coolant during hot, >85 degrees F weather). DO NOT FREEZE. The laboratory must receive specimens within 24 hours of collection.
Unacceptable Conditions:Necrotic tissue may not contain nuclei with intact DNA.
Requisition Form:Cytogenetics Lab Genetic Diagnosis Form #131
Required Information:Laboratory regulations require the following minimum information to be provided on the requisition form for a specimen to be accepted for testing: Patient name or unique identifier; date and time of collection, patient date of birth and sex, specimen type/site of collection, gestational age, test request(s), reason for referral, clinician name and UPIN/NPI, and address for reporting results. Please be certain that name/identifier on the form matches that on the specimen label.
Results include:Evidence/no evidence of trisomy 13, 18, and/or 21. Sex chromosome signal pattern.
Limitations:FISH results reflect copy number only for the loci of the probes used, and do not rule out isochromosomes or structural abnormalities of chromosomes 13, 18, 21, X, and Y.
Additional Tests Recommended: 
Additional Comments: 
Additional Tests Performed: 

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