FLT3 Mutation Analysis

FLT3 Mutation Analysis
WSLH Department: Cytogenetics
WSLH Test Code:895M60
CPT Code:81245, 81246
Includes:FLT3-ITD and FLT3-TKD (D835). FLT3 mutation testing is performed by Laboratory for Personalized Molecular Medicine (LabPMM) of San Diego, California pursuant to patents licensed from Takara Bio of Otsu, Japan.
Methodology:FLT3-ITD and FLT3-TKD mutations are detected by PCR amplification with fluorescently labeled primers and capillary electrophoresis-based fragment analysis. The FLT3-TKD PCR product is digested with EcoRV prior to electrophoresis.
Availability:Monday-Friday 7:45 AM - 4:30 PM, Saturday 7:45 AM - 12:00 PM
Turn-around Time:Approximately 10-14 days. (Reports are issued Monday-Friday 7:45 AM - 4:30 PM)
Recommended Uses:Risk stratification in patients with cytogenetically normal acute myeloid leukemia (CN-AML).
Specimen Requirements:**10 mL whole blood collected in EDTA (purple top) tube
**2-5 mL bone marrow collected in EDTA tube
**High Quality DNA (60ul) >= 1.5 ug at 25 ng/ul

Isolated nucleic acids are only accepted from a CLIA-certified laboratory or a laboratory meeting equivalent requirements.
Collection Kit/Container: 
Patient Preparation: 
Collection Instructions:Draw blood using aseptic techniques into a sterile EDTA vacuum type tube(s). Invert tube(s) to mix. If using larger tubes, draw to full volume to avoid over-treatment with anticoagulant.
Specimen Handling and Transport:Store and transport specimens at room temperature (may transport with coolant during hot, >85 degrees, weather). DO NOT FREEZE. Specimens must be received by the laboratory within 24-48 hours of collection.
Unacceptable Conditions:Blood or bone marrow that is clotted or hemolyzed is not acceptable. Blood or bone marrow must not be frozen. Plasma and serum are not acceptable.
Requisition Form:Cytogenetics Lab Neoplasia Diagnosis Form #132
Required Information:Laboratory regulations require the following minimum information to be provided on the requisition form for a specimen to be accepted for testing: Patient name or unique identifier; date and time of collection, patient date of birth and sex, specimen type/site of collection, test request(s), reason for referral, clinician name and UPIN/NPI, and address for reporting results. Please be certain that name/identifier on the form matches that on the specimen label.
Results include:*FLT3-ITD detected/not detected
*FLT3-TKD (D835) variant detected/not detected
Limitations:Analytical sensitivity/Specificity: 5 FLT3 variant containing cells in 100 total cells.
Additional Tests Recommended:*AML FISH panel, Chromosome analysis- Bone Marrow for Hematologic Disorders,
Chromosome analysis- Unstimulated Blood for Hematologic Disorders
Additional Comments: 
Additional Tests Performed: 

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