(Information current as of 07/18/2016)
The Wisconsin State Laboratory of Hygiene (WSLH) is performing polymerase chain reaction (PCR) assays for detection of Zika, dengue, and chikungunya viral nucleic acid in serum, and an IgM antigen-capture (MAC) ELISA assay for detection of Zika IgM antibody in serum. Additionally, urine specimens can now be submitted for the Zika PCR assay if they are accompanied by serum specimens. (WI Division of Public Health memo).
On July 14, 2016, the Wisconsin Department of Health Services (DHS) provided updated guidance on Zika testing – DHS Zika testing guidance memo (7/14/16)
The following information is for Wisconsin clinical and hospital laboratories.
- If a physician suspects a case of Zika virus and would like testing performed, please contact the WI Department of Health Services (WDHS) Communicable Disease Epidemiology Section (phone: 608-267-9003). Once WDHS approves testing, they will provide your laboratory with a form that must be completed and accompany the specimen. WDHS Zika virus information (look under “For Health Professionals” tab): https://www.dhs.wisconsin.gov/arboviral/zika.htm
- The WSLH is performing polymerase chain reaction (PCR) assays for detection of Zika, dengue, and chikungunya viral nucleic acid in serum, and an IgM antigen-capture (MAC) ELISA assay for detection of Zika IgM antibody in serum. Additionally, urine specimens can now be submitted for the Zika PCR assay if they are accompanied by serum specimens. NOTE: Specimens testing positive or equivocal for Zika virus IgM will still need to be forwarded to the CDC for confirmatory plaque-reduction neutralization (PRNT) testing because of the significant cross-reactivity between Zika and other Flaviviruses in the MAC ELISA assay. Also, requests for IgM testing for dengue and chikungunya viruses will continue to be sent to CDC.
- Specimen – Instructions for specimen collection and submission are provided at time of testing approval by WDHS.
- Transport – Specimens approved for testing by WDHS may be transported to the WSLH by calling Statmedex for pick-up. Specimens should be triple packaged as a Category B Biological Substance.
- Fee for Service Testing – No fee-for-service testing is being offered at this time. ALL Zika virus test requests must be approved by WDHS by calling 608-267-9003.
Zika virus has been added to the list of notifiable diseases/conditions. The WSLH will report any confirmed cases of Zika virus to WDHS.
For information on Zika virus infection:
WI Department of Health Services: https://www.dhs.wisconsin.gov/arboviral/zika.htm
Pan American Health Organization (PAHO): http://www.paho.org/hq/index.php?option=com_topics&view=article&id=427&Itemid=41484&lang=en