Legionella Clinical Culture

Legionella Clinical Culture
WSLH Department: CDD - Communicable Disease Division
WSLH Test Code:MP00420
CPT Code:87081
Price:$84.00 as of 1/1/23
Includes:Isolation and identification of Legionella species.
Methodology:Bacterial culture and identification using real-time PCR for species and serogrouping / MALDI-TOF
Availability:Monday - Friday
Turn-around Time:7-14 days
Recommended Uses:To support diagnosis of Legionnaire's Disease in the acute phase of illness.
Contraindications:This test is for clinical use only, not for use with environmental samples.
Specimen Requirements:Lung tissue, pleural fluid, transtracheal aspirate, lower respiratory secretions (sputum, bronchial wash etc.)
Collection Kit/Container:TB Sputum/Legionella
Patient Preparation:Collect pea-sized piece of tissue and/or 5-30 ml of secretions. Add a small amount of sterile distilled water to lung tissue if necessary to prevent drying. Do not use sterile saline for specimen collection as Legionella sp. are inhibited by saline.
Collection Instructions: 
Specimen Handling and Transport:Specimens should be held at 2-8C and tissues should not be allowed to dry out.
Transport with a cool pack.
Unacceptable Conditions: 
Requisition Form:CDD Requisition Form A
Required Information:Laboratory regulations require the following minimum information to be provided on the requisition form for a specimen to be accepted for testing: Patient name or unique identifier; date and time of collection, patient date of birth and sex, specimen type/site of collection, test request(s), clinician name and UPIN, and address for reporting results. Please be certain that name/identifier on the form matches that on the specimen label.
Results include: 
Limitations:Lung tissue and transtracheal aspirate have the highest yield of positive results. Sputum and pleural fluid have a lower yield. This test is not for environmental samples.
Additional Tests Recommended:Legionella urine antigen
Additional Comments:Legionella urine antigen sent to a reference lab.
Additional Tests Performed: 

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