Legionella RT-PCR Test

Legionella RT-PCR Test
WSLH Department: CDD - Communicable Disease Division
WSLH Test Code:MP00441
CPT Code:87541, 2x 87798
Price:$159.00 as of 1/1/23
Includes:Real-time PCR for the detection of Legionella species and serogrouping of Legionella pneumophila from bacterial isolates and primary cultures.
Methodology:Real-time PCR
Turn-around Time:1-3 days
Recommended Uses:Detection of Legionella DNA in bacterial isolates and primary cultures for identification and/or confirmation; species identification and serogrouping of isolates for epidemiological purposes.
Contraindications:This test is for clinical use only, not for use with environmental samples.
Specimen Requirements:Pure bacterial isolate of suspected Legionella sp. on BCYE agar.
Sputum or BAL of suspect patients collected in sterile container.
Collection Kit/Container:TB Sputum/Legionella
Patient Preparation: 
Collection Instructions: 
Specimen Handling and Transport:Isolates can be stored at ambient temperatures; avoid freezing. Primary specimens can be stored at 2-8C until transport and transported with a cool pack. Primary specimens must be tested within 3 days of collection per CDC guidance. If specimens will not be tested for 3 or more days, they should be stored at -20°C until ready for shipment and shipped on dry ice.
Unacceptable Conditions: 
Requisition Form:CDD Requisition Form A
Required Information:Laboratory regulations require the following minimum information to be provided on the requisition form for a specimen to be accepted for testing: Patient name or unique identifier; date and time of collection, patient date of birth and sex, specimen type/site of collection, test request(s), clinician name and UPIN, and address for reporting results. Please be certain that name/identifier on the form matches that on the specimen label.
Results include:Legionella DNA detected/not detected
Legionella pneumophila DNA detected/not detected
Legionella pneumophila serogroup 1 DNA detected/not detected
Limitations:This test was developed and its performance characteristics determined by the Wisconsin State Laboratory of Hygiene. It has not been cleared by the U.S. Food and Drug Administration. A negative result does not rule out Legionella infection.
Additional Tests Recommended:Legionella Culture (primary specimens); Legionella Identification (isolates)
Additional Comments:Test is not listed on the requisition form and must be written under "other tests."
Additional Tests Performed: 

Back to test list


Search Again