Isolates submitted on agar slants

Include copy of susceptibility results performed in your laboratory

• Species identification using MALDI-TOF mass spectrometry
• Antibiotic susceptibility testing via broth microdilution
• Modified Carbapenemase Inactivation Method (mCIM) to detect presence of carbapenemases
• PCR tests for presence of select resistance mechanisms when carbapenemases are detected by mCIM

Collection Kits will be provided by WSLH when testing is approved

Rectal swabs must be tested within 5 days of collection

Axilla/groin swabs should be shipped on ice and tested within 4 days for axilla/groin swabs

Tracheostomy swabs from ventilated patients. Specimens should be shipped on ice and tested within 4 days of collection

Rectal swabs from provided collection kits

Axilla/groin swabs from provided collection kits

Collection Kits will be provided by WSLH when testing is approved

Rectal swabs must be tested within 5 days of collection

Axilla/groin swabs should be shipped on ice and tested within 4 days of collection

Tracheostomy swabs from ventilated patients. Specimens should be shipped on ice and tested within 4 days of collection

Isolates submitted on agar slants

Include copy of susceptibility results performed in your laboratory

Pseudomonas aeruginosa isolates that are resistant to a carbapenem (doripenem, imipenem, meropenem) and non-susceptible to cefepime and/or ceftazadime

NOTE: Other species may be accepted on a case by case basis

• Species identification using MALDI-TOF mass spectrometry
• Antibiotic susceptibility testing via broth microdilution
• Modified Carbapenemase Inactivation Method (mCIM) to detect presence of carbapenemases
• PCR tests for presence of select resistance mechanisms when carbapenemases are detected by mCIM

• Any confirmed or suspected Candida auris isolates (See what species auris is commonly misidentified as)
• Candida glabrata from normally sterile sites (including serial glabrata isolates from patients receiving antifungal treatment
• Multi-drug resistant Candida isolates
• Unusual Candida species (any species other than albicans, C. parapsilosis, C. dubliniensis, C. lusitaniae, C. tropicalis, C. krusei)
• Any Candida species that was unable to be identified after a validated method was attempted

• Species identification using MALDI-TOF mass spectrometry
• Antifungal susceptibility testing via broth microdilution (azoles and echinocandins)
• Amphotericin B susceptibility testing using Etest strips
• Testing performed for surveillance. MIC results provided upon request.

Candida auris is a reportable condition in Wisconsin under the provision for ‘Any detection of, or illness caused by, an agent that is foreign, exotic or unusual to Wisconsin, and that has public health implications’ in Chapter DHS 145.

Please report any confirmed cases to the Local Health Department and the Wisconsin HAI Program at 608-266-0915 or 608-267-7711

• PCR
• Selective enrichment broth to isolate Candida auris
• Species identification using MALDI-TOF mass spectrometry

Isolates submitted on agar slants

Include copy of susceptibility results performed in your laboratory

Part of ARLN’s targeted surveillance project

Acinetobacter baumannii is not included in state-wide surveillance. WSLH solicits isolates from southeast WI as part of a targeted surveillance program.

If you have isolates that have been deemed highly resistant at the clinical lab or are suspected of being part of an outbreak and are not located in SE WI, contact WSLH for guidance on submission.

• Species identification using MALDI-TOF mass spectrometry
• Antibiotic susceptibility testing via broth microdilution
• PCR tests for presence of carbapenemases

Isolates submitted on agar slants

Include copy of susceptibility results performed in your laboratory

Yes

Submit completed AR Expanded AST Request Form (Excel file) for approval to:

• Email: wiarln@slh.wisc.edu
• Fax: 1-844-390-6233

• Test non-susceptible to all beta-lactams, including either ceftazidime-avibactam or meropenem-vaborbactam.

-OR-

• Enterobacteriaceae possessing NDM, VIM, or IMP genes confirmed by a molecular test and are highly resistant to all or the majority of antimicrobial agents already tested.

wiarln@slh.wisc.edu

1-800-862-1013