Use of Test

Evaluation of brushings for premalignant, malignant or inflammatory changes. Includes brushings obtained from esophagus, bronchus, stomach, small bowel, large bowel, kidney, bladder .

Patient Preparation

As per clinician


Using NGYN ThinPrep® vial:

  • Label ThinPrep® vial with patient’s full legal name, DOB, DOS, and specimen type.
  • Vigorously roll the brush along the sides and bottom of the vial.

Alternate slide method:

  • Label slides with patient’s full legal name, DOB, DOS, and specimen type..
  • Smear brush onto clean, dry slide.
  • Fix immediately in 95% ethanol or with cytology spray fixative.
  • Allow slides to fix in 95% ethanol for at least 15 minutes. Then allow slides to completely air dry.
  • Place fixed slides into slide container.

WSLH cytology requisition form #141 must include:

  • Patient’s full legal name
  • Clinic medical record number, if available
  • Date of birth
  • Date of collection
  • Specimen source
  • Number of slides submitted, if applicable
  • Clinic, clinician name and phone number
  • Pertinent clinical history
  • Payment information

Diagnostic Range

  • Negative for malignant cells
  • Atypical cells
  • Suspicious for malignancy
  • Positive for malignancy
  • Nondiagnostic/unsatisfactory


Poorly fixed or air-dried samples impair evaluation. For some specimen types, a minimum number of locally expected cells must be present to determine adequacy. Without minimum numbers, the specimen may be rendered as non-diagnostic or unsatisfactory. The presence of abnormal cells will always be documented on the report regardless of the presence or absence of local cells.



Turnaround Time

24 hours

PLEASE NOTE: Results may be delayed or the sample rejected if pertinent and/or required information needs clarification or is missing.

Test Codes

93801    BRUSH

Specimen Submission Requirements

Brushing Kit #16 and Mailers – Instructions For Submitting Specimens For Non-gynecologic Evaluations. This kit is intended for collection and shipment of brushing (non-gynecologic) specimens for analysis of premalignant or malignant disease.


  • Frosted-end glass slides (upon request)
  • NGYN ThinPrep® vial
  • Biohazard bag
  • Absorbent cloth
  • Plastic slide holder
  • Styrofoam mailers
  • Mailing labels
  • Biohazard sticker

Mandatory Requirements

  • Write patient’s full legal name on ThinPrep® container or glass slide as applicable
  • Complete WSLH requisition form #141
  • Collect specimen and transfer to NGYN ThinPrep® vial or to glass slide as applicable

Mailing Guidelines

  • Insert glass slide in plastic slide holder
  • Label holder with full legal name of patient
  • Place the NGYN ThinPrep® vial and/or plastic holder into the biohazard bag
  • Place biohazard bag and requisition orders into styrofoam container
  • Place completed requisition form in the styrofoam mailer
  • Label the styrofoam mailer with the following:
    • Your laboratory/clinic’s address
    • WSLH Cytology Department address
    • Diagnostic specimen label
    • Biohazard sticker

PLEASE NOTE: Transport ASAP or within 12 hours. The specimen does not need to be refrigerated or packed with ice. The styrofoam mailer does not need to be placed in a biohazard bag.