How to Collect a Blood Specimen

 

Guides for Specimen Collection

 

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Quick Tips for Proper Specimen Collection

Directions are also on back of the blood collection card. (Reference: Blood Collection on Filter Paper for Newborn Screening Programs, Approved Standard; 6th Edition, CLSI Document NBS01-A6)

1. Filling Out the Card (see this page for more detailed information)
  • Always use a ballpoint pen
  • Please PRINT clearly
  • To prevent specimen contamination, do not touch any of the filter paper circles, and always place the card on a clean surface.
  • The following pieces of information are critical and necessary for testing and should be included on all card submissions:
    • Birth date and time
    • Collection date and time
    • Birth weight
    • Gestational age
    • Mother’s name
    • Name of the baby’s primary care physician
  • If hearing or heart screening results are not available before the blood screening is completed, leave those fields blank. Hearing results can be submitted separately through the We-Trac queue.
2. Blood Collection
  • To prevent specimen contamination, do not touch any of the filter paper circles before or after collection.
  • Keep heel in horizontal position (heel down) at or below heart level and warm the heel to stimulate blood flow.
  • Select puncture site and cleanse with 70% isopropanol.
  • Use a sterile, disposable lancet (1.0 mm incision depth and 2.5 mm incision length) to puncture the medial or lateral plantar surface of the foot.
  • Wipe away first blood drop.
  • Allow a second LARGE blood drop to form and apply to surface of filter paper circle.
  • Fill all FIVE required circles from only one side of the filter paper.
  • Once completed, observe both sides of the filter paper to ensure complete saturation.
  • Dry specimen at room temperature 3 hours in HORIZONTAL position.
  • Once dry, inspect the card for any evidence of poor or unsatisfactory specimen collection such as:
    • blood clots
    • incomplete saturation
    • layering of the blood
    • plasma separation from the red blood cells
    • contamination of card
    • damage to filter paper
    • blood on the tan cover
  • Forward all specimens to the WSLH within 24 hours after collection.
    • NEVER BATCH SAMPLES
    • NEVER hold completed blood card for hearing or heart screening results.
  • Specimens older than seven (7) days from collection date are unsatisfactory for testing, and  a repeat collection will be required.
  • Upon receipt, the WSLH will review specimen quality. Any specimen deemed unsatisfactory for testing will be rejected, and a repeat collection will be required.
3. Specimen Evaluation at Collection Site
  • If problems occur during specimen collection, or if the specimen appears to be unsatisfactory, please recollect a specimen as soon as possible. Write CREDIT across the unsatisfactory card (see example) and send it, together with the re-collected card, to the laboratory within 24 hours after collection.
4. Specimen Evaluation at the Laboratory

The Newborn Screening Laboratory evaluates all specimens for acceptability. Those not acceptable are reported as Unsatisfactory. When a specimen is deemed Unsatisfactory for testing:

  • The WSLH notifies the submitter.
  • Submitter is responsible for recollection.
  • Repeat collection is expected within SEVEN days after notification.
5. Specimen Collection Using Capillary Tubes

The usage of capillary tubes is not recommended; however, a proper collection can be obtained if the following steps are taken:

  • Use a sterile, plain, anticoagulant-free capillary tube.
  • Use a fresh capillary tube for each circle to be filled on the card.
  • Empty contents of the tube quickly on the center of one circle to avoid clotting.
  • Do not scratch, color-in, or repeatedly dab the filter paper with the capillary tube.
6. Additional Resources
  • Blood Collection on Filter Paper for Newborn Screening Programs, Approved Standard; 6th Edition, CLSI Document NBS01-A6
  • Good Laboratory Practices for Biochemical Genetics Testing and Newborn Screening for Inherited Metabolic Disorders (April 6, 2012), Morbidity and Mortality Weekly Report (MMWR), Centers for Disease Control and Prevention
  • NBSqualityreport@slh.wisc.edu
  • Instructional video based on CLSI Standard LA4-A5 (exits WSLH site). NOTE: Appearance of newborn screening collection card and some test information in the video is different from Wisconsin’s program.